Linder E
J Invest Dermatol. 1982 Feb;78(2):116-20. doi: 10.1111/1523-1747.ep12505778.
By exposing frozen sections of human skin to fresh normal human serum, binding and activation of complement was observed. Attached complement components Clq, C4, and C3 were detected by immunofluorescence microscopy using anticomplement conjugates. Isolated Clq bound to the same structures as serum Clq, C4, and C3 and binding of C4 and C3 was dependent on binding of Clq. The complement components bound to capillary endothelial cells and to fibrillar structures in the dermis and reacted with the epidermis. Complement binding dermal fibrillar structures in adult skin were scant but abundant in fetal skin. They had similar distribution as "microfibrils" demonstrable by human autoantibodies. Antibody independence of the Clq binding was shown using isolated Clq and by the observed constant and reproducible binding of complement of normal sera lacking antibodies to the described target structures. The observations suggest that antibody independent binding of complement should be considered as a possible mechanism leading to dermal complement deposition in vivo.
通过将人皮肤冰冻切片暴露于新鲜正常人血清中，观察到补体的结合与激活。使用抗补体结合物通过免疫荧光显微镜检测附着的补体成分C1q、C4和C3。分离出的C1q与血清中的C1q、C4和C3结合于相同结构，且C4和C3的结合依赖于C1q的结合。补体成分结合于毛细血管内皮细胞以及真皮中的纤维状结构，并与表皮发生反应。成人皮肤中结合补体的真皮纤维状结构稀少，但在胎儿皮肤中丰富。它们与人类自身抗体可证实的“微原纤维”分布相似。使用分离出的C1q以及观察到缺乏抗体的正常血清补体对所述靶结构持续且可重复的结合，表明C1q结合不依赖抗体。这些观察结果提示，补体的抗体非依赖性结合应被视为体内导致真皮补体沉积的一种可能机制。
