An evaluation of suitable functions for the insulin standard curve.

The insulin radioimmunoassay technique used for human insulin has been modified for measuring rabbit insulin (i) at lower concentrations of insulin in plasma, and (ii) more precisely. Eleven algebraic functions were fitted in turn to fifty-three data sets. The goodness-of-fit was assessed in each case. It was concluded that a quadratic equation was the best function for the standard curve of the modified immunoassay, although this function is not necessarily the best for other different immunoassays.