Williams D C, Van Frank R M, Muth W L, Burnett J P
Science. 1982 Feb 5;215(4533):687-9. doi: 10.1126/science.7036343.
Escherichia coli that has been genetically manipulated by recombinant DNA technology to synthesize human insulin polypeptides (A chain, B chain, or proinsulin) contains prominent cytoplasmic inclusion bodies. The amount of inclusion product within the cells corresponds to the quantity of chimeric protein formed by the bacteria. At peak production, the inclusion bodies may occupy as much as 20 percent of the Escherichia coli cellular volume.
通过重组DNA技术进行基因操作以合成人胰岛素多肽（A链、B链或胰岛素原）的大肠杆菌含有明显的细胞质包涵体。细胞内包涵体产物的量与细菌形成的嵌合蛋白的量相对应。在产量高峰期，包涵体可能占据大肠杆菌细胞体积的20%之多。
