Eremin V A, Chekulaeva L N, Kharat'ian E F, Ostrovskiĭ D N
Mikrobiologiia. 1978 Jul-Aug;47(4):629-36.
The object of this work was to prepare deuterated growth media and to adapt Micrococcus lysodeikticus to a medium containing deuterated-substituted organic substances and deuterium oxide instead of water. M. lysodeikticus was grown on a medium prepared from the "deuterated-cells" of Chlorella, and was capable of absorbing selectively protons from such a medium containing high concentrations of deuterium. Its deuterated cells ("monsters") produced structures consisting of several (up to 8) smaller cells, angular in shape and having a thicker (2--3 times) cell wall. Apparently, adaptation to a deuterated medium is accompanied with changes in the cell wall biosynthesis as a result of which the separation of daughter cells is interfered with in the course of cell division, and the cells are more resistant to the action of lysozyme.
这项工作的目的是制备氘代生长培养基,并使溶壁微球菌适应一种含有氘代取代有机物质和氧化氘而非水的培养基。溶壁微球菌在由小球藻的“氘代细胞”制备的培养基上生长,并且能够从这种含有高浓度氘的培养基中选择性地吸收质子。其氘代细胞(“怪物”)产生由几个(多达8个)较小细胞组成的结构,形状呈角形,细胞壁更厚(2至3倍)。显然,对氘代培养基的适应伴随着细胞壁生物合成的变化,其结果是在细胞分裂过程中子细胞的分离受到干扰,并且细胞对溶菌酶的作用更具抗性。