从球状芽孢杆菌中纯化和鉴定一种小DNA结合蛋白HB

Purification and characterisation of a small DNA-binding protein, HB, from Bacillus globigii.

作者信息

Imber R, Bächinger H, Bickle T A

出版信息

Eur J Biochem. 1982 Mar 1;122(3):627-32. doi: 10.1111/j.1432-1033.1982.tb06485.x.

DOI:10.1111/j.1432-1033.1982.tb06485.x

Abstract

We have purified a small, heat-stable DNA-binding protein from Bacillus globigii: HB protein. The protein binds cooperatively to double-stranded DNA and the DNA-protein complexes are destabilised by the presence of moderate (0.1 M) levels of monovalent cations or by low levels (10 mM) of Mg2+. We have also purified two small DNA-binding proteins from Escherichia coli, NS1 and NS2 [Suryanarayana, T. and Subramanian, A.R. (1978) Biochim. Biophys. Acta, 520, 342-357], also known as HU protein [Rouvière-Yaniv, J. and Gros, F. (1975) Proc. Natl Acad. Sci. USA, 72, 3428-3432] that have been extensively studied by others. We have prepared antibodies against all three proteins and have shown that all three are, by immunological criteria, related.

摘要

我们从球状芽孢杆菌中纯化出了一种小的、热稳定的DNA结合蛋白：HB蛋白。该蛋白与双链DNA协同结合，并且DNA-蛋白质复合物会因中等浓度（0.1M）的单价阳离子或低浓度（10mM）的Mg2+的存在而变得不稳定。我们还从大肠杆菌中纯化出了两种小的DNA结合蛋白，NS1和NS2 [苏里亚纳拉亚纳，T. 和苏布拉马尼亚姆，A.R.（1978年）《生物化学与生物物理学报》，520，342 - 357]，它们也被称为HU蛋白 [鲁维耶 - 亚尼夫，J. 和格罗斯，F.（1975年）《美国国家科学院院刊》，72，3428 - 3432]，其他人对其进行了广泛研究。我们制备了针对这三种蛋白的抗体，并通过免疫学标准表明这三种蛋白是相关的。