Slavin S, Seidel H J
Exp Hematol. 1982 Feb;10(2):206-16.
The hemopoietic status was examined in the blood, bone marrow and spleen of (BALB/c X C57BL/6)F1 hybrid mice treated with fractionated total lymphoid irradiation (TLI), with or without reconstitution with 30 X 10(6) syngeneic or parental C57BL/6 marrow cells. Colony forming units of pluripotent (CFUS) and granuloid committed stem cells (CFUC) were assayed in non-irradiated (femur) and irradiated bone marrow (humerus), and in spleen 9, 18, 73 and 109 days following TLI. Recovery of CFUS and CFUC was nearly complete as early as day 18, and fully recovered on day 73 in endogenously as well as exogenously (syngeneic and semiallogeneic) reconstituted mice. Mice transplanted with C57BL/6 bone marrow exhibited a 10-fold increase of CFUC in the spleen on day 9 and maintained a 4-fold increase of CFUC on day 109, with normal CFUC in shielded, and near normal CFUC in irradiated bones. In contrast, the number of CFUS in the irradiated humerus was below 10% of all controls on days 18-73 and below 30% on day 109. Also, the number of CFUS in the spleen was very much reduced at days 73-109. Unirradiated femora contained normal CFUC and only mildly depressed CFUS. The data suggest that repopulation of bone marrow is adequate although somewhat less efficient in irradiated as compared to shielded bones, whether or not exogenous marrow is transplanted. Semiallogeneic chimeras (C57BL/6 leads to F1) show low CFUS relative to high CFUC levels, particularly in irradiated organs (spleen and humerus). This may suggest an advantage, from a hemapoietic standpoint, to selective irradiation of lymphoid tissues, rather than the less selective wide fields that overlap marrow containing bones.
对接受分次全身淋巴照射（TLI）的（BALB/c×C57BL/6）F1杂交小鼠的血液、骨髓和脾脏的造血状态进行了检查，这些小鼠在有或没有用30×10⁶同基因或亲代C57BL/6骨髓细胞进行重建的情况下接受照射。在未照射的（股骨）和照射后的骨髓（肱骨）以及TLI后9、18、73和109天的脾脏中检测多能集落形成单位（CFUS）和粒细胞定向干细胞（CFUC）。CFUS和CFUC的恢复早在第18天就几乎完成，并且在第73天内源性以及外源性（同基因和半同种异体）重建的小鼠中完全恢复。移植了C57BL/6骨髓的小鼠在第9天脾脏中的CFUC增加了10倍，在第109天CFUC保持4倍的增加，在屏蔽的小鼠中CFUC正常，在照射的骨骼中CFUC接近正常。相比之下，在第18 - 73天，照射的肱骨中的CFUS数量低于所有对照组的10%，在第109天低于30%。此外，在第73 - 109天，脾脏中的CFUS数量也大幅减少。未照射的股骨含有正常的CFUC，CFUS仅轻度降低。数据表明，无论是否移植外源性骨髓，骨髓的重新填充是足够的，尽管与屏蔽的骨骼相比，照射后的骨髓重新填充效率略低。半同种异体嵌合体（C57BL/6→F1）相对于高CFUC水平显示出低CFUS水平，特别是在照射的器官（脾脏和肱骨）中。从造血的角度来看，这可能表明选择性照射淋巴组织比重叠含骨髓骨骼的选择性较低的广泛区域具有优势。
