一种耐热性溶菌性内肽酶的分子量和氨基酸组成
Molecular weight and amino acid composition of a thermostable lytic endopeptidase.
作者信息
Turkington E O, Welker N E
出版信息
J Bacteriol. 1982 Apr;150(1):418-20. doi: 10.1128/jb.150.1.418-420.1982.
Abstract
A thermostable lytic endopeptidase from Bacillus stearothermophilus 1503-4R was purified 14,500-fold, with a 34% recovery of lytic activity. The enzyme is a basic protein (pI, 9.7) with a molecular weight of 15,100 and is composed of approximately 129 amino acid residues.
摘要
从嗜热脂肪芽孢杆菌1503 - 4R中纯化出一种耐热性溶菌性内肽酶，纯化倍数达14,500倍，溶菌活性回收率为34%。该酶是一种碱性蛋白质（pI为9.7），分子量为15,100，由大约129个氨基酸残基组成。
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本文引用的文献
1
The spectrophotometric determination of tyrosine and tryptophan in proteins.蛋白质中酪氨酸和色氨酸的分光光度测定法。
Biochem J. 1946;40(5-6):628-32. doi: 10.1042/bj0400628.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
Purification and properties of a thermophilic bacteriophage lytic enzyme.一种嗜热噬菌体裂解酶的纯化及特性
J Virol. 1967 Jun;1(3):617-25. doi: 10.1128/JVI.1.3.617-625.1967.
5
Structure of the cell wall of Bacillus stearothermophiluys: mode of action of a thermophilic bacteriophage lytic enzyme.嗜热脂肪芽孢杆菌细胞壁结构：一种嗜热噬菌体裂解酶的作用模式
J Bacteriol. 1971 Sep;107(3):697-703. doi: 10.1128/jb.107.3.697-703.1971.
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