Klages D, Meyer I, Schwartz W, Näveke R
Z Allg Mikrobiol. 1981;21(10):729-37.
Besides leaching of sulfide ores with thiobacilli, a second way of microbial leaching is tested with carbon-heterotrophic fungi and bacteria for those types of ores, where thiobacilli fail to work. The active agents are metabolic products, f. i. organic acids, which are released into the medium producing water soluble compounds with heavy metals. A screening method is described for recognizing active strains in samples of soil, metal-containing sites, mining heaps and other biocoenoses, using the dilution method in plates with different media. The critical signs of supposed leaching activity are halo formations around growing colonies in turbid agar media with suspended particles of Ca carbonate or Ca phosphates of low solubility, and in a second group of experiments with insoluble compounds of heavy metals, f. i. oxides. The leaching activity was tested with metal compounds suspended in liquid media and inoculated with active strains of the screening program. First results were reached with oxides of copper (CuO), uranium (U3O8) and an uranium-containing phosphorite.
除了用硫杆菌浸出硫化矿外，还对碳异养真菌和细菌进行了微生物浸出的第二种方法的测试，用于硫杆菌无法发挥作用的那些矿石类型。活性剂是代谢产物，例如有机酸，它们释放到培养基中，与重金属形成水溶性化合物。描述了一种筛选方法，用于识别土壤、含金属场地、矿堆和其他生物群落样本中的活性菌株，该方法在含有不同培养基的平板中使用稀释法。假定浸出活性的关键迹象是在含有碳酸钙或低溶解度磷酸钙悬浮颗粒的浑浊琼脂培养基中，生长菌落周围形成晕圈，以及在第二组使用重金属不溶性化合物（例如氧化物）的实验中。用悬浮在液体培养基中并接种筛选程序活性菌株的金属化合物测试浸出活性。首先用氧化铜（CuO）、氧化铀（U₃O₈）和一种含铀磷矿得到了结果。
