Weinstein R, Hoover G A, Majure J, van der Spek J, Stemerman M B, Maciag T
J Cell Physiol. 1982 Jan;110(1):23-8. doi: 10.1002/jcp.1041100105.
The hormones which support growth, in vitro, of normal, neonatal human foreskin fibroblasts were determined. Whereas thrombin and hydrocortisone were major growth stimulants, platelet-derived growth factor was not. Human foreskin fibroblasts grew in a serum-free, biochemically defined medium consisting of epidermal growth factor (100 ng/ml), insulin (100 ng/ml), transferrin 10 micrograms/ml), thrombin (1 microgram/ml), ascorbic acid (10 micrograms/ml), and hydrocortisone (5 x 10(-5) M) in a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12, supplemented with ovalbumin (1 mg/ml) and trace elements. The growth achieved was comparable to that achieved with 5% fetal bovine serum. Neither platelet-derived growth factor, fibroblast growth factor, nor somatomedin activity increased proliferation. This serum-free medium, designated Defined Medium F, provides a biochemically defined system for growth and limited subcultivation of human foreskin fibroblasts in vitro.
我们确定了在体外支持正常新生儿人类包皮成纤维细胞生长的激素。凝血酶和氢化可的松是主要的生长刺激剂，而血小板衍生生长因子则不是。人类包皮成纤维细胞在一种无血清、化学成分明确的培养基中生长，该培养基由表皮生长因子（100纳克/毫升）、胰岛素（100纳克/毫升）、转铁蛋白（10微克/毫升）、凝血酶（1微克/毫升）、抗坏血酸（10微克/毫升）和氢化可的松（5×10⁻⁵摩尔）组成，存在于杜尔贝科改良伊格尔培养基和哈姆F-12的1:1混合物中，并添加了卵清蛋白（1毫克/毫升）和微量元素。所实现的生长与使用5%胎牛血清时相当。血小板衍生生长因子、成纤维细胞生长因子或生长介素活性均未增加增殖。这种无血清培养基被命名为限定培养基F，它为体外人类包皮成纤维细胞的生长和有限传代提供了一个化学成分明确的系统。
