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通过吲哚斑点试验对呼吸道分离培养物中的嗜血杆菌属进行鉴别。

Differentiation of Haemophilus spp. in Respiratory isolate cultures by an indole spot test.

作者信息

Welch D F, Ahlin P A, Matsen J M

出版信息

J Clin Microbiol. 1982 Feb;15(2):216-9. doi: 10.1128/jcm.15.2.216-219.1982.

Abstract

Indole spot tests using isolated, nonhemolytic colonies of Haemophilus species were positive for 90 of 151 (60%) respiratory isolates of Haemophilus influenzae, whereas 67 to 72 (93%) isolates of H. influenzae from cerebrospinal fluid and blood specimens were indole positive. Only 4 of 117 (3%) Haemophilus parainfluenzae isolates were positive for indole spot tests. Thus, indole-positive, nonhemolytic Haemophilus isolates in respiratory cultures can be presumptively identified as H. influenzae.

摘要

使用流感嗜血杆菌分离出的非溶血菌落进行吲哚斑点试验,结果显示,151株呼吸道流感嗜血杆菌分离株中有90株(60%)呈阳性,而从脑脊液和血液标本中分离出的67至72株(93%)流感嗜血杆菌吲哚试验呈阳性。117株副流感嗜血杆菌分离株中只有4株(3%)吲哚斑点试验呈阳性。因此,呼吸道培养中吲哚试验呈阳性的非溶血嗜血杆菌分离株可初步鉴定为流感嗜血杆菌。

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本文引用的文献

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Haemophilus aphrophilus infections: clinical and bacteriologic studies.嗜沫嗜血杆菌感染:临床与细菌学研究
Ann N Y Acad Sci. 1970 Oct 30;174(2):468-87. doi: 10.1111/j.1749-6632.1970.tb45574.x.
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Evaluation of two spot-indole reagents.两种斑点吲哚试剂的评估
Appl Microbiol. 1969 Jun;17(6):923-4. doi: 10.1128/am.17.6.923-924.1969.
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A rapid method for the differentiation of Haemophilus strains. The porphyrin test;一种区分嗜血杆菌菌株的快速方法。卟啉试验;
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):835-42. doi: 10.1111/j.1699-0463.1974.tb02381.x.

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