Velasquez M T, Wan S H, Barr J W, Maronde R F
J Clin Pharmacol. 1981 Nov-Dec;21(11):657-62. doi: 10.1002/j.1552-4604.1981.tb05679.x.
In a randomized crossover trial in six normal male subjects, the effect of pretreatment with probenecid on natriuresis and renin release in response to bumetanide was studied. The subjects received 120 mEq sodium and 80 mEq potassium per day. A single dose of 2 mg bumetanide was administered on the fourth morning after pretreatment with either placebo or probenecid. Creatinine and uric acid were measured in serum and urine, plasma renin activity was determined by radioimmunoassay of angiotensin I, and plasma and urine concentrations of bumetanide were measured by a highly sensitive radioimmunoassay method. Probenecid reduced both natriuresis and hyperreninemia induced by bumetanide. This effect is postulated to be due not to a direct action on sodium excretion but is probably secondary to inhibition of renal tubular secretion of bumetanide. Consequently, these findings appear to support the concept that the quantity of bumetanide delivered to the tubular lumen is an important determinant of its diuretic effect.
在一项针对6名正常男性受试者的随机交叉试验中，研究了丙磺舒预处理对布美他尼所致利钠作用和肾素释放的影响。受试者每天摄入120 mEq钠和80 mEq钾。在接受安慰剂或丙磺舒预处理后的第四个早晨，给予单剂量2 mg布美他尼。测定血清和尿液中的肌酐和尿酸，通过放射免疫分析法检测血管紧张素I来确定血浆肾素活性，并采用高灵敏度放射免疫分析法测定布美他尼的血浆和尿液浓度。丙磺舒降低了布美他尼所致的利钠作用和高肾素血症。推测此效应并非由于对钠排泄的直接作用，而可能继发于对布美他尼肾小管分泌的抑制。因此，这些发现似乎支持这样的观点，即输送到肾小管管腔的布美他尼量是其利尿作用的重要决定因素。
