Sleytr U B, Robards A W
J Microsc. 1982 Apr;126(Pt 1):101-22. doi: 10.1111/j.1365-2818.1982.tb00361.x.
Freeze-fracture and freeze-etching techniques do not provide artefact-free images of native in vivo or in vitro cells and tissues. Each preparation stage can produce specific artefacts which must be recognized and understood if these methods are to contribute meaningful information to cell biology, This paper reviews the latest information available on artefacts in freeze-fracture replication (and etching) methods and points to possibilities for avoiding some of them. Different specimens show different sensitivity to artefactual changes and the final images must be interpreted carefully with regard to the multi-event process that has led to their production.
冷冻断裂和冷冻蚀刻技术无法提供天然活体内或体外细胞及组织的无伪像图像。每个制备阶段都会产生特定的伪像，如果要让这些方法为细胞生物学提供有意义的信息，就必须认识和理解这些伪像。本文综述了冷冻断裂复制（和蚀刻）方法中有关伪像的最新信息，并指出了避免其中一些伪像的可能性。不同的标本对伪像变化的敏感度不同，最终图像必须根据导致其产生的多步骤过程进行仔细解读。
