[Contacts of Escherichia coli RNA polymerase subunits with nucleotides of lacUV5 promoter].

We have localized contacts between DNA and subunits of E. coli RNA polymerase (of both holo and core enzymes) along lacUV5 promoter. In the complex with the holo enzyme the anti-sense strand of DNA makes contacts with beta' subunit at -47, -46, -30, -12, +1, +5, +7, +9; beta subunit at -30, -25, -23, -12, +11, +30 and delta subunit at -17, -5, -3 nucleotides, while the sense-strand of DNA makes contacts with beta' subunit at -22, -21, -9, -6, -4, +3, +4, +6, +17; beta subunit at +17, +25, +27, +34; and delta subunit at -35, -18 nucleotides. In the complex with the core enzyme the anti-sense strand of DNA makes contacts with beta'subunit at -47, -23, -5, -3, +5, +7, +9; beta subunit at -23, -16, +11, +30 nucleotides while the sense-strand of DNA makes contacts with beta' subunit at -20, -19, +3, +4, +6, +17; beta subunit at -36, -35, -34, -31, -29, +17, +25, +27, +34 nucleotides alpha subunits of the holo as well as the core enzyme show no contact with DNA in the conditions providing specific complex formation.