Deeg H J, Storb R, Shulman H M, Weiden P L, Graham T C, Thomas E D
Transplantation. 1982 Apr;33(4):443-6. doi: 10.1097/00007890-198204000-00021.
Marrow transplants were carried out between unrelated DLA-nonidentical dogs. Recipients were conditioned for transplantation by total body irradiation (TBI) given eigher as a single dose of 9 Gy (900 rad) or fractionated in three increments of 6 Gy (600 rad) each at intervals of 48 hr. All recipients received marrow, less than or equal to 4 x 10(8) cells/kg, and no buffy coat cells. No immunosuppression was given after grafting. All 10 dogs given single dose total body irradiation failed to show engraftment and died with marrow aplasia and infectious complications (median survival 12 days). In contrast, all 10 dogs given fractionated TBI had sustained engraftment and died with graft-versus-host disease (GVHD) and infectious complications (median survival 12.5 days). None of the dogs died from radiation-induced gastroenteritis. In conclusion, resistance to DLA-nonidentical unrelated marrow grafts can be abrogated by high-dose TBI. This technique may allow hemopoietic engraftment even after i vitro manipulation of the marrow such as lymphocyte depletion by cell separation or treatment with anti-T cell antisera.
在不相关的DLA不匹配犬之间进行了骨髓移植。接受者通过全身照射（TBI）进行移植预处理，全身照射要么单次给予9 Gy（900拉德），要么分三次给予，每次6 Gy（600拉德），间隔48小时。所有接受者均接受了小于或等于4×10⁸个细胞/千克的骨髓，且未接受富含白细胞层细胞。移植后未给予免疫抑制。所有10只接受单次全身照射的犬均未显示植入，并死于骨髓再生障碍和感染性并发症（中位生存期12天）。相比之下，所有10只接受分次全身照射的犬均实现了持续植入，并死于移植物抗宿主病（GVHD）和感染性并发症（中位生存期12.5天）。没有一只犬死于辐射性胃肠炎。总之，高剂量全身照射可消除对DLA不匹配无关骨髓移植物的抗性。即使在对骨髓进行体外操作（如通过细胞分离去除淋巴细胞或用抗T细胞抗血清处理）之后，该技术也可能实现造血细胞植入。
