Krasnov I B
Tsitologiia. 1982 Feb;24(2):230-2.
A method of fixation by the Carnoy liquid and of gallocyanine staining is proposed in order to obtain clear-cut boundaries of the neuron body, nucleus and nucleolus in cryostat sections of non-fixed frozen nerve tissue, for subsequent morphometry of the body and nucleus of nerve cells. In the resulting sections, the clearness of boundaries of the body and nucleus in a neuron is sufficient for measuring its diameter with the coefficient of variation not exceeding 2.5%.
提出了一种用卡诺伊液固定和用镓氰宁染色的方法，以便在未固定的冷冻神经组织的低温切片中获得神经元胞体、细胞核和核仁的清晰边界，用于后续神经细胞胞体和细胞核的形态测量。在所得切片中，神经元胞体和细胞核边界的清晰度足以测量其直径，变异系数不超过2.5%。
