Kirshner J J, Goldberg J, Nelson D A, Gottlieb A J
Am J Med. 1982 Apr;72(4):615-19. doi: 10.1016/0002-9343(82)90462-4.
A double-layer agar culture assay for granulopoietic progenitor cell (CFU-C) was employed to study the in vitro growth pattern of bone marrow or peripheral blood cells from 37 patients at diagnosis of acute nonlymphocytic leukemia. The patients were classified as having type A growth if there were greater than 50 colonies (A1), or greater than one colony and greater than 50 clusters was observed after 14 days in culture (A2). Type B growth was defined as no growth (B1), less than 50 colonies and less than 50 clusters (B2) or cluster growth only (B3). Agar cultures containing marrow cells from 10 patients were stained in situ, and the colonies and clusters revealed abnormal cellular maturation. Treatment with cytosine arabinoside and an anthracycline resulted in a complete remission of leukemia in one of 15 patients with type A growth and 17 of 22 patients with type B growth. The subtype of growth pattern did not correlate with the mechanism (i.e., drug resistance versus kinetic resistance) for the failure to achieve a complete remission. The subtype of type B growth (B1, B2 or B#) did not correlate with the duration of complete remission. Nonetheless, the growth patterns observed in cultures of cells obtained from patients with acute nonlymphocytic leukemia appear to be excellent predictors of the success of achieving complete remission with induction therapy.
采用双层琼脂培养法检测粒细胞祖细胞（CFU-C），以研究37例急性非淋巴细胞白血病患者在诊断时骨髓或外周血细胞的体外生长模式。若培养14天后观察到大于50个集落（A1），或大于1个集落且大于50个细胞簇（A2），则将患者分类为具有A型生长。B型生长定义为无生长（B1）、少于50个集落且少于50个细胞簇（B2）或仅为细胞簇生长（B3）。对含有10例患者骨髓细胞的琼脂培养物进行原位染色，集落和细胞簇显示细胞成熟异常。用阿糖胞苷和蒽环类药物治疗后，15例A型生长患者中有1例、22例B型生长患者中有17例白血病完全缓解。生长模式亚型与未能实现完全缓解的机制（即耐药性与动力学耐药性）无关。B型生长的亚型（B1、B2或B3）与完全缓解持续时间无关。尽管如此，急性非淋巴细胞白血病患者细胞培养中观察到的生长模式似乎是诱导治疗实现完全缓解成功与否的良好预测指标。
