Shih L Y, Dunn P, Liaw S J
Acta Haematol. 1986;76(1):20-4. doi: 10.1159/000206012.
The growth pattern of marrow cells in agar culture was studied in 90 adult patients with acute nonlymphocytic leukemia (ANLL) at diagnosis. We classified the abnormal growth patterns into 4 groups, A: no growth, B: decreased growth, C: excessive microcluster formation and D: excessive cluster growth with more than 20 colonies. There was a good correlation between growth pattern and FAB subtype. A predominance of group A growth was observed in M1, while group B growth was found in 50% of patients with M2 and M5. No relationships between the growth patterns and other clinical parameters were detected. Sixty-six patients were evaluable for treatment outcome. The growth pattern significantly correlated with complete remission rate. The remission rates were 52, 87, 80, and 25% for patients with group A, B, C and D growth, respectively. Analyses of remission duration and survival curves showed significant differences among the different growth patterns. Patients with D growth experienced a shorter remission duration and a lower survival rate than other groups. These results indicate that the in vitro culture growth pattern in untreated ANLL is of prognostic significance in predicting the response to therapy.
对90例初诊的成年急性非淋巴细胞白血病（ANLL）患者的骨髓细胞在琼脂培养中的生长模式进行了研究。我们将异常生长模式分为4组，A组：无生长；B组：生长减少；C组：过度微集落形成；D组：有超过20个集落的过度集落生长。生长模式与FAB亚型之间存在良好的相关性。在M1中观察到A组生长占优势，而在50%的M2和M5患者中发现B组生长。未检测到生长模式与其他临床参数之间的关系。66例患者可评估治疗结果。生长模式与完全缓解率显著相关。A、B、C和D组生长的患者缓解率分别为52%、87%、80%和25%。缓解持续时间和生存曲线分析显示不同生长模式之间存在显著差异。D组生长的患者缓解持续时间较短，生存率低于其他组。这些结果表明，未经治疗的ANLL体外培养生长模式在预测治疗反应方面具有预后意义。
