[Horseradish peroxidase: kinetic studies and optimization of the activity determination with the substrates H2O2 and o-phenylenediamine (author's transl)].

To optimize the determination of the activity of peroxidase, an enzyme often used as a marker and indicator system in enzyme immunoassays, the kinetics of the catalytic reaction and its dependence on the H2O2 and o-phenylenediamine concentration at various pH values were investigated. In addition, the influence of buffer systems, the reaction temperature, and the reaction time were determined. Based on the results, a test procedure is proposed for the measurement of peroxidase down to concentrations of 2 ng/l.