Gallati H, Brodbeck H
J Clin Chem Clin Biochem. 1982 Apr;20(4):221-5.
To optimize the determination of the activity of peroxidase, an enzyme often used as a marker and indicator system in enzyme immunoassays, the kinetics of the catalytic reaction and its dependence on the H2O2 and o-phenylenediamine concentration at various pH values were investigated. In addition, the influence of buffer systems, the reaction temperature, and the reaction time were determined. Based on the results, a test procedure is proposed for the measurement of peroxidase down to concentrations of 2 ng/l.
为优化过氧化物酶活性的测定，过氧化物酶是酶免疫分析中常用的一种标记物和指示系统，研究了催化反应的动力学及其在不同pH值下对过氧化氢和邻苯二胺浓度的依赖性。此外，还测定了缓冲体系、反应温度和反应时间的影响。基于这些结果，提出了一种用于测量低至2 ng/l浓度过氧化物酶的测试程序。
