咖啡因对大肠杆菌核苷酸库的影响。
Effect of caffeine on nucleotide pools in Escherichia coli.
作者信息
Sandlie I, Kleppe K
出版信息
Chem Biol Interact. 1982 Jun;40(2):141-8. doi: 10.1016/0009-2797(82)90096-5.
The influence of caffeine on the intracellular concentration of various nucleoside triphosphates in addition do dTMP and dTDP, in Escherichia coli has been investigated. For most of the nucleoside triphosphates the presence of 10 mM caffeine in the medium resulted in a small increase in pool size 5 min after addition, followed by a slow decrease to the initial concentration. In the case of dTTP, however, the pool size reached a maximum, 2-fold higher than the initial value, 30 min after caffeine addition. This increase in dTTP level is probably due to an effect of caffeine on the DNA synthesis process and synthesis of dTDP-sugars.
除了dTMP和dTDP外，还研究了咖啡因对大肠杆菌中各种核苷三磷酸细胞内浓度的影响。对于大多数核苷三磷酸而言，培养基中存在10 mM咖啡因会导致添加后5分钟内池大小略有增加，随后缓慢降至初始浓度。然而，在dTTP的情况下，添加咖啡因30分钟后，池大小达到最大值，比初始值高2倍。dTTP水平的这种增加可能是由于咖啡因对DNA合成过程和dTDP-糖合成的影响。
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