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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Comp Biochem Physiol B. 1982;71(4):663-8. doi: 10.1016/0305-0491(82)90478-3.

Digestive fluid proteases of the silkworm, 6B1-3, were separated, partially purified and their properties were compared. 2. These proteases were different in the substrate specificity, effect of inhibitors, Km and influence of Mn2+. 3. Hydrolyzing ability for natural substrates was comparatively high in 6B1, whereas the hydrolysis of synthetic substrates of trypsin by 6B1 was lower than that by 6B2 or 3. 4. The protease activity was sensitive to DFP and PMSF. The soybean trypsin inhibitor differentially affected three proteases. Silkworm haemolymph strongly inhibited the protease activity of 6B2 and 3, but scarcely affected 6B1.

对家蚕的消化液蛋白酶6B1 - 3进行了分离、部分纯化并比较了它们的性质。2. 这些蛋白酶在底物特异性、抑制剂的作用、米氏常数（Km）以及锰离子（Mn2 +）的影响方面存在差异。3. 6B1对天然底物的水解能力相对较高，而6B1对胰蛋白酶合成底物的水解能力低于6B2或6B3。4. 蛋白酶活性对二异丙基氟磷酸（DFP）和苯甲基磺酰氟（PMSF）敏感。大豆胰蛋白酶抑制剂对三种蛋白酶的影响不同。家蚕血淋巴强烈抑制6B2和6B3的蛋白酶活性，但对6B1几乎没有影响。

Comp Biochem Physiol B. 1982;71(4):663-8. doi: 10.1016/0305-0491(82)90478-3.

Digestive fluid proteases of the silkworm, 6B1-3, were separated, partially purified and their properties were compared. 2. These proteases were different in the substrate specificity, effect of inhibitors, Km and influence of Mn2+. 3. Hydrolyzing ability for natural substrates was comparatively high in 6B1, whereas the hydrolysis of synthetic substrates of trypsin by 6B1 was lower than that by 6B2 or 3. 4. The protease activity was sensitive to DFP and PMSF. The soybean trypsin inhibitor differentially affected three proteases. Silkworm haemolymph strongly inhibited the protease activity of 6B2 and 3, but scarcely affected 6B1.

对家蚕的消化液蛋白酶6B1 - 3进行了分离、部分纯化并比较了它们的性质。2. 这些蛋白酶在底物特异性、抑制剂的作用、米氏常数（Km）以及锰离子（Mn2 +）的影响方面存在差异。3. 6B1对天然底物的水解能力相对较高，而6B1对胰蛋白酶合成底物的水解能力低于6B2或6B3。4. 蛋白酶活性对二异丙基氟磷酸（DFP）和苯甲基磺酰氟（PMSF）敏感。大豆胰蛋白酶抑制剂对三种蛋白酶的影响不同。家蚕血淋巴强烈抑制6B2和6B3的蛋白酶活性，但对6B1几乎没有影响。