Lewis L, Verna J M, Levinstone D, Sher S, Marek L, Bell E
J Cell Sci. 1982 Feb;53:21-36. doi: 10.1242/jcs.53.1.21.
Translocation of human fibroblasts in culture was studied using techniques of time-lapse cinemicrography, indirect immunofluorescence, and computer analysis. An inverse relationship between the velocity of cells during the last hour of life and the density of stress fibers seen by immune staining was demonstrated. Translocating cells generally assumed one of two interconvertible morphologies: a triangular tailed shape or tailed fibroblast (TF), and a tailless form that resembled a half-moon, which we call a half-moon fibroblast (HMF). The tail of TFs formed only on regions of substrate that had been previously traversed by cells. The half-moon morphology developed either on previously used or on virgin substrate. Cells adopted the HMF rather than the TF morphology with a four-fold greater frequency. HMFs translocated slightly faster than TFs. The foregoing observation suggest that the fibroblast tail is not an organelle essential for translocation. Since our technique allowed us to distinguish between cells which were cycling and those which had left cycle, we compared their velocities and found them to be similar. Also the average velocities of cells of different population-doubling levels (10th, 30th, 40th) were approximately equal.
运用延时显微电影摄影技术、间接免疫荧光技术和计算机分析方法，对培养中的人成纤维细胞的迁移进行了研究。结果表明，细胞生命最后一小时的速度与免疫染色观察到的应力纤维密度呈负相关。迁移的细胞通常呈现两种可相互转换的形态之一：一种是三角形尾状形态或尾状成纤维细胞（TF），另一种是类似半月形的无尾形态，我们称之为半月形成纤维细胞（HMF）。TF的尾部仅在细胞先前穿过的底物区域形成。半月形形态在先前使用过的底物或未使用过的底物上均可形成。细胞呈现HMF形态的频率比呈现TF形态的频率高四倍。HMF的迁移速度比TF略快。上述观察结果表明，成纤维细胞的尾部不是迁移所必需的细胞器。由于我们的技术使我们能够区分处于增殖周期的细胞和已脱离增殖周期的细胞，我们比较了它们的速度，发现它们相似。不同群体倍增水平（第10代、第30代、第40代）的细胞平均速度也大致相等。
