Tilahun G, Stockdale P H
J Protozool. 1982 Feb;29(1):129-32. doi: 10.1111/j.1550-7408.1982.tb02895.x.
The sensitivity and specificity of the indirect fluorescent antibody (IFA) test for the detection of serum antibodies were examined in mice that were infected with Eimeria falciformis, E. ferrisi, E. papillata, or E. vermiformis. For the study of each species, five groups of mice were given graded inoculation doses of 10, 10(2), 10(3), 10(4), or 10(5) sporulated oocysts in a primary infection. The sixth group was infected with three sequential doses of 1.5 x 10(3), 1.5 x 10(4), and 1.5 x 10(5) sporulated oocysts per mouse at two- to three-week intervals. All groups of infected mice developed serum antibodies. Sera were titrated by the IFA test with purified sporozoites. Strong fluorescence and high IFA titers were observed with homologous reactions mainly with the sera from mice infected with the higher inoculation dose levels in primary infections and from those given three sequential inoculation doses. Immunological cross reaction among the four species of Eimeria occurred at dilutions of 1:10 to 1:160. Very weak or no fluorescence of free sporozoites was observed with sera from noninfected mice, and there was no fluorescence of sporozoites contained in intact sporocysts.
在感染了镰形艾美耳球虫、费氏艾美耳球虫、乳头艾美耳球虫或蠕虫状艾美耳球虫的小鼠中，检测了间接荧光抗体（IFA）试验检测血清抗体的敏感性和特异性。对于每种艾美耳球虫的研究，在初次感染时，将五组小鼠分别接种10、10²、10³、10⁴或10⁵个孢子化卵囊的分级剂量。第六组小鼠每隔两到三周依次接种三剂，每只小鼠接种1.5×10³、1.5×10⁴和1.5×10⁵个孢子化卵囊。所有感染组的小鼠都产生了血清抗体。用纯化的子孢子通过IFA试验对血清进行滴定。在同源反应中观察到强荧光和高IFA滴度，主要是来自初次感染中接种较高剂量水平的小鼠以及接受三次连续接种剂量的小鼠的血清。四种艾美耳球虫之间的免疫交叉反应发生在1:10至1:160的稀释度下。未感染小鼠的血清对游离子孢子观察到非常弱的荧光或无荧光，完整子孢子囊内的子孢子也无荧光。
