Maguen E, Azen S P, Pinhas S, Villaseñor R A, Nesburn A B
Ophthalmic Surg. 1982 Mar;13(3):217-20.
A study was carried out to evaluate effects of various sources of variation on the accuracy and reproducibility of the modified artificial chamber in obtaining lamellar sections from rabbit cornea. Sources of variation included a) measurement error, inter-cornea and inter-session variation, b) length of storage time M-K medium, and c) choice of preselected values of thickness and diameter. The findings indicated tha a) measurement error, inter-cornea, and inter-session variations were of nominal magnitude; b) the length of storage time up to 10 days did not effect the precision of the procedure; c) the precision of diameter determinations did not depend on the preselected value of the diameter; d) the precision of thickness determinations did depend on preselected parameter values; and e) parameter values tended to be underestimated. Regression equations therefore are provided to adjust for the underestimation of thickness and diameter. Based on these findings, it was concluded that the modified artificial anterior chamber is adequate for use in experimental refractive keratoplasty since it provides adequate diamter values and is reproducible.
开展了一项研究，以评估各种变异来源对改良人工房在从兔角膜获取板层切片时的准确性和可重复性的影响。变异来源包括：a）测量误差、角膜间和实验环节间的变异；b）在M-K培养基中的储存时间长度；c）厚度和直径预选值的选择。研究结果表明：a）测量误差、角膜间和实验环节间的变异程度较小；b）长达10天的储存时间不会影响该操作的精度；c）直径测定的精度不取决于直径的预选值；d）厚度测定的精度确实取决于预选参数值；e）参数值往往被低估。因此，提供了回归方程以校正厚度和直径的低估。基于这些发现，得出结论：改良人工前房适用于实验性屈光性角膜成形术，因为它能提供足够的直径值且具有可重复性。
