接种鼠伤寒沙门氏菌的鸡的白细胞迁移抑制
Leukocyte migration inhibition in chickens inoculated with Salmonella typhimurium.
作者信息
Nagaraja K V, Newman J A, Pomeroy B S
出版信息
Am J Vet Res. 1982 May;43(5):916-8.
Leukocyte migration-inhibition tests were conducted in chickens infected with Salmonella typhimurium. A relatively new and simple method that involves the migration of leukocytes under agarose gel was used. Migration distance was projected onto a white background at 40X magnification, using a microprojector. Quantitation of migration was done by measuring the linear distance the cells had moved from the margin of the agarose wells. The details of the technique and the importance of it in studies of cell-mediated immune response are discussed. Chickens inoculated with S typhimurium had a significant leukocyte migration inhibition.
对感染鼠伤寒沙门氏菌的鸡进行了白细胞迁移抑制试验。采用了一种相对较新且简单的方法，该方法涉及白细胞在琼脂糖凝胶下的迁移。使用微型投影仪，在40倍放大倍数下将迁移距离投射到白色背景上。通过测量细胞从琼脂糖孔边缘移动的线性距离来进行迁移定量。讨论了该技术的细节及其在细胞介导免疫反应研究中的重要性。接种鼠伤寒沙门氏菌的鸡出现了显著的白细胞迁移抑制。
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