Ashley M P, Kotlarski I
Aust J Exp Biol Med Sci. 1982 Feb;60(Pt 1):1-21. doi: 10.1038/icb.1982.1.
In vivo and in vitro parameters of tumour resistance were examined after immunization of mice with the attenuated 11RX strain of S. enteritidis. During the bacterial carrier state produced by intraperitoneal (i.p.) or intravenous (i.v.) injection of 11RX the mice were resistant to i.p. tumour growth, could destroy i.p. injected 125I- or 131I-labelled tumour cells in vivo and had non-specifically cytotoxic peritoneal cells (PC) which could lyse 51Cr-labelled tumour cells in vitro. Most of the in vivo and in vitro cytotoxic activity could be attributed to activated macrophages (La Posta et al., 1982). The predominantly local nature of 11RX-induced anti-tumour activity was indicated by the superiority of the i.p. route of infection for induction of tumour resistance and in vivo and in vitro cytotoxicity. After i.v. injection of 11RX, none of the anti-tumour effects outlasted the bacterial carrier state. However, after i.p. infection, a dichotomy was observed between in vitro and in vivo anti-tumour effects. In vitro PC cytotoxicity lasted only for the length of the 11RX carrier state (approximately 30 days), whereas resistance to i.p. tumour growth lasted for 60 to 100 day s and was correlated closely with cytotoxic activity measured in vivo. Possible reasons for this dichotomy are discussed.
用肠炎沙门氏菌减毒11RX菌株免疫小鼠后，检测了肿瘤抗性的体内和体外参数。在通过腹腔内（i.p.）或静脉内（i.v.）注射11RX产生的细菌携带状态期间，小鼠对腹腔内肿瘤生长具有抗性，能够在体内破坏腹腔内注射的125I或131I标记的肿瘤细胞，并且具有可在体外裂解51Cr标记的肿瘤细胞的非特异性细胞毒性腹膜细胞（PC）。体内和体外的大部分细胞毒性活性可归因于活化的巨噬细胞（La Posta等人，1982年）。11RX诱导的抗肿瘤活性主要是局部性质，这一点通过腹腔内感染途径在诱导肿瘤抗性以及体内和体外细胞毒性方面的优越性得以体现。静脉注射11RX后，没有一种抗肿瘤作用能超过细菌携带状态。然而，腹腔内感染后，在体外和体内抗肿瘤作用之间观察到了二分法。体外PC细胞毒性仅在11RX携带状态期间（约30天）持续，而对腹腔内肿瘤生长的抗性持续60至100天，并且与体内测量的细胞毒性活性密切相关。讨论了这种二分法的可能原因。
