Sostero D, Cecconato E, Boniolo A, Callegaro L
Boll Ist Sieroter Milan. 1981;60(6):508-12.
Antibodies against HBsAg were immobilized by covalent linkage on nylon beads to be used in solid phase enzyme-linked immunoassays. The covalent bond between the protein and the partially hydrolyzed support was obtained via glutaraldehyde or succinylester methods. These particular solid phases were compared, in a conventional enzyme-linked immunosorbent assay for hepatitis B antigen, with polystyrene beads coated by simple adsorption of IgG on plastic surface. The analytical response was more satisfactory when the polystyrene beads were used as immunoextractive support even if more IgG molecules could be immobilized on nylon beads.
将抗乙肝表面抗原（HBsAg）抗体通过共价连接固定在尼龙珠上，用于固相酶联免疫分析。蛋白质与部分水解载体之间的共价键通过戊二醛或琥珀酸酯法获得。在用于检测乙型肝炎抗原的传统酶联免疫吸附试验中，将这些特定的固相与通过简单吸附IgG于塑料表面而包被的聚苯乙烯珠进行了比较。即使更多的IgG分子能够固定在尼龙珠上，但使用聚苯乙烯珠作为免疫提取支持物时，分析响应更令人满意。
