Yamamoto J K, Blalock J E, Johnson H M
Eur J Immunol. 1982 Mar;12(3):222-7. doi: 10.1002/eji.1830120310.
Unstimulated human peripheral lymphocytes were cytotoxic for normal mouse spleen cells and suppressed the in vitro antibody plaque-forming cell (PFC) response of these cells to sheep red blood cells and dinitrophenylated Ficoll. The cells in the lymphocyte population that were responsible for the immunosuppression had properties of natural killer (NK) or NK-like cells in that they were: (a) non-E-rosetting, (b) nonadherent, (c) unaffected by treatment with anti-human immunoglobulin plus complement, (d) cytotoxic against an established human NK target, K562 leukemic cells, and (e) partially inactivated by mitomycin C. Addition of the human NK-like cells to mouse spleen cell cultures at the time of antigen addition and at an effector cell to target cell ratio as low as 0.67:1 resulted in 85 to 96% suppression of the PFC response. Addition of NK-like cells to cultures 18 h before harvesting in 5-day cultures required higher concentrations and ratios (2.7:1) of effector to target cells to significantly suppress the PFC response. The data suggest that human NK-like activity in suppression of the mouse PFC response is due to killing of the targets. The mouse spleen cell PFC system represents a potential model for assessment of human NK activity that is quite dramatic in its effect and can be used in addition to the well known 51Cr-release assay. Also, since the mouse spleen cell is a normal cell, it provides a model in the PFC system for studying the mechanism of NK regulation of normal cellular function. An additional finding of this study was the observation that E-rosetting T cells significantly enhanced the mouse PFC response. Thus, human peripheral lymphocytes contain discrete cellular population that either enhance or suppress the mouse PFC response.
未受刺激的人外周淋巴细胞对正常小鼠脾细胞具有细胞毒性，并抑制这些细胞对绵羊红细胞和二硝基苯基化菲可的体外抗体空斑形成细胞（PFC）反应。淋巴细胞群体中负责免疫抑制的细胞具有自然杀伤（NK）或NK样细胞的特性，即它们：（a）不形成E花环，（b）不黏附，（c）不受抗人免疫球蛋白加补体处理的影响，（d）对已建立的人NK靶标K562白血病细胞具有细胞毒性，以及（e）被丝裂霉素C部分灭活。在添加抗原时，以低至0.67:1的效应细胞与靶细胞比例将人NK样细胞添加到小鼠脾细胞培养物中，导致PFC反应受到85%至96%的抑制。在5天培养物收获前18小时将NK样细胞添加到培养物中，需要更高的效应细胞与靶细胞浓度和比例（2.7:1）才能显著抑制PFC反应。数据表明，人NK样活性对小鼠PFC反应的抑制是由于靶细胞的杀伤。小鼠脾细胞PFC系统代表了一种评估人NK活性的潜在模型，其效果非常显著，除了众所周知的51Cr释放试验外还可使用。此外，由于小鼠脾细胞是正常细胞，它在PFC系统中提供了一个研究NK对正常细胞功能调节机制的模型。本研究的另一个发现是观察到形成E花环的T细胞显著增强了小鼠PFC反应。因此，人外周淋巴细胞包含离散的细胞群体，它们要么增强要么抑制小鼠PFC反应。
