Homma H, Nojima S
J Biochem. 1982 Apr;91(4):1103-10. doi: 10.1093/oxfordjournals.jbchem.a133792.
When 2-[14C]acyl lysophosphatidylethanolamine was incubated with the envelope fraction of E. coli in the presence of Mg2+ ion, phosphatidylethanolamine, acylphosphatidylglycerol and free fatty acid were produced. When 2-[14C]acyl lysophosphatidylglycerol was examined similarly, six phospholipids as well as free fatty acid were produced. These were dilysocardiolipin, lysocardiolipin, phosphatidylglycerol, cardiolipin, bis(monoacylglycero)phosphate and acylphosphatidylglycerol; they were identified by thin layer chromatography, acetolysis and mild alkaline hydrolysis. Studies with an E. coli mutant which is deficient in cardiolipin synthase showed that dilysocardioilipin, lysocardiolipin and cardiolipin were synthesized by cardiolipin synthase. Bis(monoacylglycero)phosphate as well as acylphosphatidylglycerol was produced by acylphosphatidylglycerol synthase. While phosphatidylglycerol and cardiolipin were produced predominantly from 2-acyl lysophosphatidylglycerol, almost the same amounts of dilysocardiolipin, lysocardiolipin and bis(monoacylglycero)phosphate were produced from the 1-acyl and 2-acyl isomers. Metabolites of 2-[14C]acyl lysophosphatidic acid were also examined. Phosphatidic acid, acylphosphatidylglycerol, free fatty acid and monoglyceride were produced, together with a small amount of diglyceride.
当2-[¹⁴C]酰基溶血磷脂酰乙醇胺在Mg²⁺离子存在下与大肠杆菌的包膜组分一起温育时，会产生磷脂酰乙醇胺、酰基磷脂酰甘油和游离脂肪酸。当以类似方式检测2-[¹⁴C]酰基溶血磷脂酰甘油时，会产生六种磷脂以及游离脂肪酸。它们是二赖氨酸心磷脂、溶血心磷脂、磷脂酰甘油、心磷脂、双（单酰甘油）磷酸酯和酰基磷脂酰甘油；通过薄层色谱、乙酰解和温和碱性水解对它们进行了鉴定。对缺乏心磷脂合酶的大肠杆菌突变体的研究表明，二赖氨酸心磷脂、溶血心磷脂和心磷脂是由心磷脂合酶合成的。双（单酰甘油）磷酸酯以及酰基磷脂酰甘油是由酰基磷脂酰甘油合酶产生的。虽然磷脂酰甘油和心磷脂主要由2-酰基溶血磷脂酰甘油产生，但1-酰基和2-酰基异构体产生的二赖氨酸心磷脂、溶血心磷脂和双（单酰甘油）磷酸酯数量几乎相同。还检测了2-[¹⁴C]酰基溶血磷脂酸的代谢产物。产生了磷脂酸、酰基磷脂酰甘油、游离脂肪酸和甘油单酯，以及少量甘油二酯。
