Neely J E, Hake D A
J Histochem Cytochem. 1982 Jun;30(6):536-7. doi: 10.1177/30.6.7047639.
To provide a constant level of tracer doses of tritiated thymidine to cultured cells during continuous infusion, miniature osmotic infusion pumps were used to provide replacement thymidine. By determining the loss of isotope from the media during nonreplacement, the rate of constant infusion replacement to maintain thymidine levels was calculated. The replacement rates were similar for the three cell lines examined and allowed a standard osmotic pump infusion.
为了在连续输注过程中向培养细胞提供恒定水平的氚标记胸腺嘧啶核苷示踪剂量，使用微型渗透输注泵来提供替代胸腺嘧啶核苷。通过测定非替代期间培养基中同位素的损失，计算出维持胸腺嘧啶核苷水平的恒定输注替代率。所检测的三种细胞系的替代率相似，并允许进行标准的渗透泵输注。
