[被包裹在磷脂囊泡中的噬菌体M13 DNA对细菌细胞的转染]
[Transfection of bacterial cells by DNA of phage M13 entrapped in phospholipid vesicles].
作者信息
Beliaev N D, Budker V G, Maksimova T G, Naumova L P, Sokolov A V
出版信息
Mol Biol (Mosk). 1982 May-Jun;16(3):612-8.
PMID:7048068
Abstract
The possibility of transfection of bacterial cells by phage M13 DNA entrapped into phospholipid vesicles (liposomes) has been studied. Two types of liposomes differing in size were used. Entrapped DNA was transferred by liposomes into Ca2+-treated E. coli cells. Efficiency of the transfection in the case of small (ca. 400 A) liposomes was 2--3 orders of magnitude higher than that of free DNA extracted from such liposomes.
摘要
对包裹于磷脂囊泡（脂质体）中的噬菌体M13 DNA转染细菌细胞的可能性进行了研究。使用了两种大小不同的脂质体。包裹的DNA通过脂质体转移至经Ca2+处理的大肠杆菌细胞中。对于小（约400埃）脂质体，转染效率比从此类脂质体中提取的游离DNA的转染效率高2至3个数量级。
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