Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography.
作者信息
Turk J, Weiss S J, Davis J E, Needleman P
出版信息
Prostaglandins. 1978 Aug;16(2):291-309. doi: 10.1016/0090-6980(78)90031-x.
PMID:704934
Abstract
Fluorescent esters of the prostaglandins D2, E2, F2alpha, and 6-keto-F1alpha and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1alpha produced by bovine aortic microsomes has been achieved with this method.
摘要
相似文献
1
Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography.
Prostaglandins. 1978 Aug;16(2):291-309. doi: 10.1016/0090-6980(78)90031-x.
2
Quantitative profiling of prostaglandins and thromboxane by high-resolution gas chromatography-selected--ion monitoring.
J Chromatogr. 1983 Nov 25;279:581-6. doi: 10.1016/s0021-9673(01)93660-x.
3
Identification of prostaglandin D2 as a major prostaglandin in homogenates of rat brain.
Prostaglandins. 1977 Oct;14(4):633-43. doi: 10.1016/0090-6980(77)90190-3.
4
High performance liquid chromatography and UV detection for the separation and quantitation of prostaglandins.用于前列腺素分离和定量的高效液相色谱法及紫外检测法。
Prostaglandins. 1981 Jan;21(1):101-12. doi: 10.1016/0090-6980(81)90200-8.
5
Separation and quantitative determination of radiolabeled prostaglandins, thromboxanes, 6-keto-prostaglandin F1 alpha and other arachidonic acid metabolites produced in biological material.生物材料中产生的放射性标记前列腺素、血栓烷、6-酮-前列腺素F1α及其他花生四烯酸代谢产物的分离与定量测定。
J Chromatogr. 1983 Jun 10;275(1):61-70. doi: 10.1016/s0378-4347(00)84344-3.
6
Determination of prostaglandins D2, E2, F2 alpha and 6-ketoprostaglandin F1 alpha as well as thromboxane B2 in rat brain by gas chromatography-mass spectrometry.采用气相色谱-质谱联用技术测定大鼠脑中前列腺素D2、E2、F2α、6-酮前列腺素F1α以及血栓素B2的含量。
J Chromatogr. 1988 Apr 29;426(2):345-50. doi: 10.1016/s0378-4347(00)81961-1.
7
Prostaglandin and thromboxane synthesis by microsomes of inflamed rabbit ciliary body--iris.炎症兔睫状体 - 虹膜微粒体合成前列腺素和血栓素
Invest Ophthalmol Vis Sci. 1981 Apr;20(4):442-9.
8
A radioimmunoassay for 6-keto-prostaglandin F1alpha.6-酮-前列腺素F1α的放射免疫测定法。
Prostaglandins. 1978 Mar;15(3):383-97. doi: 10.1016/0090-6980(78)90122-3.
9
Transformation of arachidonate into 6-oxoprostaglandin F1 alpha, thromboxane B2 and prostaglandin E2 by sheep lung microsomal fraction.绵羊肺微粒体部分将花生四烯酸转化为6-氧代前列腺素F1α、血栓素B2和前列腺素E2。
Biochem J. 1978 Feb 15;170(2):441-4. doi: 10.1042/bj1700441.
10
Prostaglandin and thromboxane production by rat decidual microsomes.大鼠蜕膜微粒体产生前列腺素和血栓素的情况。
Prostaglandins. 1977 Nov;14(5):813-7. doi: 10.1016/0090-6980(77)90298-2.
引用本文的文献
1
Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen.从单个组织样本中测量脂质介质、蛋白质和信使核糖核酸的方法。
Anal Biochem. 2015 Jan 15;469:34-42. doi: 10.1016/j.ab.2014.10.004. Epub 2014 Oct 20.
2
Ultralow concentrations of ibuprofen activate cell prostaglandin synthesis.
Appl Biochem Biotechnol. 1996 Oct-Nov;61(1-2):167-71. doi: 10.1007/BF02785699.
3
Femtomole analysis of prostaglandin pharmaceuticals.前列腺素药物的飞摩尔分析。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8315-9. doi: 10.1073/pnas.82.24.8315.
Zotero 插件