Tai H H, Yuan B, Wu A T
Biochem J. 1978 Feb 15;170(2):441-4. doi: 10.1042/bj1700441.
In the presence of haemoglobin and isoproterenol, the microsomal fraction of sheep lung catalysed the conversion of arachidonate predominantly into thromboxane B2 and to a lesser extent into 6-oxoprostaglandin F1alpha. Very little prostaglandin E2 and prostaglandin F2alpha were formed. If reduced glutathione was added in combination with haemoglobin and isoproterenol, the synthesis of prostaglandin E2 was favoured over that of thromboxane B2 and 6-oxoprostaglandin F1alpha. The identities of these products were confirmed by t.l.c. and by combined g.l.c.-mass spectrometry. These results indicate that microsomal fraction of sheep lung possesses active prostaglandin synthase, prostacyclin synthase and thromboxane synthase activities.
在血红蛋白和异丙肾上腺素存在的情况下，绵羊肺微粒体部分主要将花生四烯酸催化转化为血栓素B2，较少程度地转化为6-氧代前列腺素F1α。生成的前列腺素E2和前列腺素F2α很少。如果将还原型谷胱甘肽与血红蛋白和异丙肾上腺素一起添加，则前列腺素E2的合成比血栓素B2和6-氧代前列腺素F1α更受青睐。通过薄层层析和气相色谱-质谱联用技术确认了这些产物的身份。这些结果表明，绵羊肺微粒体部分具有活性前列腺素合酶、前列环素合酶和血栓素合酶活性。
