Fitzgerald-Hayes M, Clarke L, Carbon J
Cell. 1982 May;29(1):235-44. doi: 10.1016/0092-8674(82)90108-8.
We determined the nucleotide sequence of DNA segments containing functional centromeres (CEN3 and CEN11) isolated from yeast chromosomes III and XI. The two centromere regions differ in primary nucleotide sequence, but contain structural features in common. Both centromere regions contain an extremely A + T-rich core segment 87-88 bp in length, flanked by two short sequences (14 bp and 11 bp) that are identical in both DNAs. These elements plus one additional 10 bp region of perfect homology are positioned in an almost identical spatial arrangement within the two centromere regions. Significant homologies are also observed among the sequences flanking the high A + T region and various satellite DNA sequences from higher eucaryotes, although no repeated sequences occur near the yeast centromeres. Centromere activity in vivo is maintained on relatively small DNA fragments (627 bp for CEN3 and 858 bp for CEN11), as assayed by mitotic stabilization of autonomously replicating ars plasmids in yeast.
我们测定了从酵母第三和第十一条染色体中分离出的包含功能性着丝粒（CEN3和CEN11）的DNA片段的核苷酸序列。这两个着丝粒区域的主要核苷酸序列不同，但具有共同的结构特征。两个着丝粒区域均包含一个长度为87 - 88 bp的富含A+T的核心片段，两侧是两个短序列（14 bp和11 bp），这两个短序列在两种DNA中是相同的。这些元件加上另外一个10 bp的完全同源区域，在两个着丝粒区域内以几乎相同的空间排列定位。在富含A+T区域两侧的序列与来自高等真核生物的各种卫星DNA序列之间也观察到显著的同源性，尽管在酵母着丝粒附近没有重复序列。通过酵母中自主复制的ars质粒的有丝分裂稳定测定，体内着丝粒活性在相对较小的DNA片段上得以维持（CEN3为627 bp，CEN11为858 bp）。
