White F P, White S R
J Neurobiol. 1977 Jul;8(4):315-24. doi: 10.1002/neu.480080404.
Proteins synthesized by soma located in L4 dorsal root ganglia and supplied to the axonal branches extending centrally in the dorsal root and peripherally towards the sciatic nerve were analyzed for radioactivity following injections of [3H] leucine into the L4 dorsal root ganglia. All proteins located in the dorsal root and sciatic nerve were analyzed by SDS acrylamide gel electrophoresis at various times post injection. The differences in radioactivity between the dorsal root and sciatic nerve proteins were mainly quantitative and not qualitative, with many proteins of various molecular weight ranges being transported into both segments. Generally, it appears that in both axonal branches the high molecular weight proteins are transported at the highest rate, medium weights slower and low molecular weight proteins slowest. More proteins of high and low molecular weights are transported into the dorsal root whereas more of those of medium molecular weight are transported towards the sciatic nerve.
将[3H]亮氨酸注射到L4背根神经节中后，对位于L4背根神经节的胞体合成并供应至在背根中向中枢延伸以及向外周延伸至坐骨神经的轴突分支的蛋白质进行放射性分析。在注射后的不同时间，通过SDS聚丙烯酰胺凝胶电泳对位于背根和坐骨神经中的所有蛋白质进行分析。背根和坐骨神经蛋白质之间的放射性差异主要是定量的而非定性的，许多不同分子量范围的蛋白质被运输到两个节段中。一般来说，在两个轴突分支中，高分子量蛋白质的运输速率最高，中等分子量的较慢，低分子量蛋白质最慢。更多的高分子量和低分子量蛋白质被运输到背根中，而更多的中等分子量蛋白质被运输到坐骨神经中。
