原代培养中正常和肿瘤小鼠乳腺上皮细胞的无血清生长
Serum-free growth of normal and tumor mouse mammary epithelial cells in primary culture.
作者信息
Imagawa W, Tomooka Y, Nandi S
出版信息
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4074-7. doi: 10.1073/pnas.79.13.4074.
Abstract
Freshly isolated normal and tumor mouse mammary epithelial cells embedded within a collagen gel matrix undergo sustained growth when cultured for as long as 3 wk in a serum-free medium composed of a 1:1 (vol/vol) mixture of Hepesbuffered Ham's F12 and Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor (EGF), transferrin, bovine serum albumin fraction V, and cholera toxin. Of these additives, only insulin, EGF, and albumin are required for the growth of most normal cells. Albumin is not always an absolute requirement for growth but greatly enhances it. Lithium has been found to stimulate the growth of normal cells and can replace EGF. The collagen matrix culture system allows sustained growth of primary cultures of both normal and neoplastic mammary epithelium in serum-free conditions. This serum-free system will be useful in identifying and investigating the role of hormones, growth factors, and nutritional factors in regulating the growth of mammary epithelial cells.
摘要
新鲜分离的正常和肿瘤小鼠乳腺上皮细胞嵌入胶原凝胶基质中，当在由Hepes缓冲的Ham's F12和杜氏改良伊格尔培养基按1:1（体积/体积）混合组成的无血清培养基中培养长达3周时，会经历持续生长，该培养基补充了胰岛素、表皮生长因子（EGF）、转铁蛋白、牛血清白蛋白V组分和霍乱毒素。在这些添加剂中，大多数正常细胞生长仅需要胰岛素、EGF和白蛋白。白蛋白并非总是生长的绝对必需成分，但能极大地促进生长。已发现锂可刺激正常细胞生长并可替代EGF。胶原基质培养系统可使正常和肿瘤性乳腺上皮的原代培养物在无血清条件下持续生长。这种无血清系统将有助于鉴定和研究激素、生长因子及营养因子在调节乳腺上皮细胞生长中的作用。
相似文献
1
Serum-free growth of normal and tumor mouse mammary epithelial cells in primary culture.原代培养中正常和肿瘤小鼠乳腺上皮细胞的无血清生长
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4074-7. doi: 10.1073/pnas.79.13.4074.
2
Growth effect of lithium on mouse mammary epithelial cells in serum-free collagen gel culture.锂对无血清胶原凝胶培养中小鼠乳腺上皮细胞的生长作用。
J Cell Physiol. 1983 Dec;117(3):290-6. doi: 10.1002/jcp.1041170303.
3
Somatomedin-C substitutes for insulin for the growth of mammary epithelial cells from normal virgin mice in serum-free collagen gel cell culture.在无血清胶原凝胶细胞培养中，生长调节素C可替代胰岛素促进正常未孕小鼠乳腺上皮细胞的生长。
Endocrinology. 1986 Dec;119(6):2695-9. doi: 10.1210/endo-119-6-2695.
4
Growth of normal mouse vaginal epithelial cells in and on collagen gels.正常小鼠阴道上皮细胞在胶原凝胶中和凝胶上的生长情况。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3743-7. doi: 10.1073/pnas.80.12.3743.
5
Growth of mouse vaginal epithelial cells in culture: effect of sera and supplemented serum-free media.培养条件下小鼠阴道上皮细胞的生长：血清及添加成分的无血清培养基的影响
In Vitro Cell Dev Biol. 1987 Aug;23(8):535-40. doi: 10.1007/BF02620970.
6
Serum-free culture of enriched mouse anterior and ventral prostatic epithelial cells in collagen gel.在胶原凝胶中对富集的小鼠前列腺前叶和腹侧上皮细胞进行无血清培养。
In Vitro Cell Dev Biol. 1990 Jul;26(7):722-30. doi: 10.1007/BF02624429.
7
Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium.逗号-D小鼠乳腺上皮细胞在激素限定的无血清培养基中的生长及连续传代。
Cancer Res. 1987 Jul 15;47(14):3776-82.
8
Primary culture of normal rat mammary epithelial cells within a basement membrane matrix. I. Regulation of proliferation by hormones and growth factors.基底膜基质中正常大鼠乳腺上皮细胞的原代培养。I. 激素和生长因子对增殖的调节。
In Vitro Cell Dev Biol. 1990 Aug;26(8):791-802. doi: 10.1007/BF02623621.
9
Influence of hormone and growth factor interactions on the proliferative potential of normal rat mammary epithelial cells in vitro.激素与生长因子相互作用对体外培养的正常大鼠乳腺上皮细胞增殖潜能的影响。
J Cell Physiol. 1987 Jul;132(1):161-7. doi: 10.1002/jcp.1041320123.
10
Stimulation of mammary epithelial cell growth in vitro: interaction of epidermal growth factor and mammogenic hormones.体外刺激乳腺上皮细胞生长：表皮生长因子与乳腺生成激素的相互作用。
Endocrinology. 1985 Apr;116(4):1514-24. doi: 10.1210/endo-116-4-1514.
引用本文的文献
1
Adipo-Epithelial Transdifferentiation in In Vitro Models of the Mammary Gland.体外乳腺模型中的脂肪上皮转分化。
Cells. 2024 May 30;13(11):943. doi: 10.3390/cells13110943.
2
A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands.从鼠乳腺嵴建立的具有生成功能性乳腺能力的克隆干细胞系。
In Vitro Cell Dev Biol Anim. 2019 Dec;55(10):861-871. doi: 10.1007/s11626-019-00406-8. Epub 2019 Sep 16.
3
Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids.Wnt 和 Neuregulin1/ErbB 信号通路延长了激素反应性乳腺类器官的 3D 培养。
Nat Commun. 2016 Oct 26;7:13207. doi: 10.1038/ncomms13207.
4
Cellular microenvironment influences the ability of mammary epithelia to undergo cell cycle.细胞微环境影响乳腺上皮细胞进行细胞周期的能力。
PLoS One. 2011 Mar 29;6(3):e18144. doi: 10.1371/journal.pone.0018144.
5
An in vitro model of epithelial cell growth stimulation in the rodent mammary gland.啮齿动物乳腺上皮细胞生长刺激的体外模型。
Cell Prolif. 2003 Aug;36(4):177-90. doi: 10.1046/j.1365-2184.2003.00265.x.
6
Genetic manipulation of mammary epithelium by transplantation.通过移植对乳腺上皮进行基因操作。
J Mammary Gland Biol Neoplasia. 1996 Jan;1(1):75-89. doi: 10.1007/BF02096304.
7
The proliferation of mouse mammary epithelial cells in response to specific mitogens is modulated by the mammary fat pad in vitro.在体外，小鼠乳腺上皮细胞对特定促细胞分裂剂的增殖受到乳腺脂肪垫的调节。
In Vitro Cell Dev Biol Anim. 1998 May;34(5):385-92. doi: 10.1007/s11626-998-0020-2.
8
Reconstruction of neural tube-like structures in vitro from primary neural precursor cells.利用原代神经前体细胞在体外重建神经管样结构。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9683-7. doi: 10.1073/pnas.90.20.9683.
9
Serum-free primary culture of normal mouse mammary epithelial and stromal cells.正常小鼠乳腺上皮细胞和基质细胞的无血清原代培养。
In Vitro Cell Dev Biol Anim. 1994 Dec;30A(12):859-66. doi: 10.1007/BF02639396.
10
Cell proliferation and milk protein gene expression in rabbit mammary cell cultures.兔乳腺细胞培养中的细胞增殖和乳蛋白基因表达
J Cell Biol. 1983 May;96(5):1435-42. doi: 10.1083/jcb.96.5.1435.
本文引用的文献
1
Identification of somatomedin-like polypeptides produced by mammary tumors of BALB/c mice.BALB/c小鼠乳腺肿瘤产生的类生长调节素多肽的鉴定。
Cancer Res. 1980 Dec;40(12):4368-72.
2
Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol.用白蛋白、转铁蛋白、铁、不饱和脂肪酸、卵磷脂和胆固醇完全替代促红细胞生成素依赖性红细胞前体（CFU-E）原代培养中的血清。
Exp Cell Res. 1980 Mar;126(1):121-6. doi: 10.1016/0014-4827(80)90476-0.
3
Effects of hormones and growth factors on human mammary epithelial cells in collagen gel culture.激素和生长因子对胶原凝胶培养的人乳腺上皮细胞的影响。
Cancer Res. 1981 Mar;41(3):1021-7.
4
Multiple hormone interactions in the developmental biology of the mammary gland.乳腺发育生物学中的多种激素相互作用。
Physiol Rev. 1980 Oct;60(4):1049-106. doi: 10.1152/physrev.1980.60.4.1049.
5
Initiation of DNA synthesis in mammary epithelium and mammary tumors by lithium ions.锂离子引发乳腺上皮细胞和乳腺肿瘤中的DNA合成。
J Cell Physiol. 1980 Apr;103(1):41-6. doi: 10.1002/jcp.1041030107.
6
Methods for growth of cultured cells in serum-free medium.在无血清培养基中培养细胞的方法。
Anal Biochem. 1980 Mar 1;102(2):255-70. doi: 10.1016/0003-2697(80)90151-7.
7
An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes.一种控制表皮生长的内分泌方法：人角质形成细胞的无血清培养。
Science. 1981 Mar 27;211(4489):1452-4. doi: 10.1126/science.6970413.
8
Epidermal growth factor requirement for development of cultured mammary gland.培养乳腺发育对表皮生长因子的需求。
Nature. 1980 May 22;285(5762):250-2. doi: 10.1038/285250a0.
9
Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels.包埋于胶原凝胶中的正常乳腺上皮细胞原代培养中的持续生长。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2088-92. doi: 10.1073/pnas.77.4.2088.
10
Interaction of unilamellar liposomes with serum lipoproteins and apolipoproteins.单层脂质体与血清脂蛋白和载脂蛋白的相互作用。
J Lipid Res. 1980 Nov;21(8):993-1003.
Zotero 插件