Tomooka Y, Imagawa W, Nandi S, Bern H A
J Cell Physiol. 1983 Dec;117(3):290-6. doi: 10.1002/jcp.1041170303.
The effect of lithium on the growth of mammary epithelial cells from adult virgin and midpregnant BALB/c or BALB/cfC3H mice was tested in a serum-free collagen gel culture system. The serum-free medium consisted of a 1:1 mixture of Ham's F12 and Dulbecco's Modified Eagle's medium supplemented with insulin, transferrin, cholera toxin, epidermal growth factor (EGF), and bovine serum albumin fraction V (BSA V). A multifold increase in cell number occurred during 10-12 days of culture in this medium. In dose-response studies in which the concentration of each component of this serum-free medium was varied in turn, the addition of LiCL (10 mM) enhanced growth at most concentrations of each factor. However, LiCl could not enhance growth in the absence of insulin or BSA V, but could replace EGF. The optimal concentration of LiCl was 5-10 mM; higher concentrations (20-80 mM) were toxic. KCl (1-10 mM) when added to the serum-free medium slightly stimulated growth; the addition of NaCl to the medium had little effect on growth. LiCl did not enhance the growth of cells from spontaneous mammary tumors of BALB/cfC3H mice.
在无血清胶原凝胶培养系统中，检测了锂对成年未孕及妊娠中期BALB/c或BALB/cfC3H小鼠乳腺上皮细胞生长的影响。无血清培养基由Ham's F12和杜尔贝科改良伊格尔培养基按1:1混合而成，并添加了胰岛素、转铁蛋白、霍乱毒素、表皮生长因子（EGF）和牛血清白蛋白V组分（BSA V）。在这种培养基中培养10 - 12天期间，细胞数量出现了数倍的增加。在剂量反应研究中，依次改变这种无血清培养基各成分的浓度，添加LiCl（10 mM）在每个因子的大多数浓度下都能促进生长。然而，在没有胰岛素或BSA V的情况下，LiCl不能促进生长，但可以替代EGF。LiCl的最佳浓度为5 - 10 mM；更高浓度（20 - 80 mM）具有毒性。将KCl（1 - 10 mM）添加到无血清培养基中可轻微刺激生长；向培养基中添加NaCl对生长几乎没有影响。LiCl不能促进BALB/cfC3H小鼠自发性乳腺肿瘤细胞的生长。
