心肌损伤：通过抗肌球蛋白荧光微球启动的细胞分选进行定量分析。

Myocardial injury: quantitation by cell sorting initiated with antimyosin fluorescent spheres.

作者信息

Khaw B A, Scott J, Fallon J T, Cahill S L, Haber E, Homcy C

出版信息

Science. 1982 Sep 10;217(4564):1050-3. doi: 10.1126/science.7051286.

Abstract

Spheres coated with antibodies specific for myosin were used to detect myocardial cell membrane disruption by scanning electron microscopy. Injury in a population of cultured myocytes as then followed and measured by fluorescence-activated cell sorting. This approach provides a unique method for quantitating the evolution of myocardial injury and potentially for assessing the efficacy of interventions aimed at myocardial protection.

摘要

用包被有针对肌球蛋白特异性抗体的球体，通过扫描电子显微镜检测心肌细胞膜的破坏情况。然后通过荧光激活细胞分选对一群培养的心肌细胞的损伤情况进行跟踪和测量。这种方法为定量心肌损伤的演变以及潜在地评估旨在心肌保护的干预措施的疗效提供了一种独特的方法。