Acetylcholine system in the isolated ventral and dorsal horn neurons from bovine spinal cord.

We have isolated the ventral and dorsal horn neurons from the bovine spinal cord using our isolation procedure and determined choline acetyltransferase, acetylcholinesterase and choline uptake activity in these neurons. Choline acetyltransferase and choline uptake activity were higher in the ventral than in the dorsal horn neurons, showing their cell specificity to some extent, but acetylcholinesterase activity did not show any differences between the two cell types.