Macoviak J A, Stephenson L W, Ochs R, Edmunds L H
Chest. 1982 Jan;81(1):78-81. doi: 10.1378/chest.81.1.78.
Pleurodesis with a sclerosing agent was attempted in an animal model in which a pneumothorax with an active air leak was created. Adult rabbits had a small left thoracotomy and then a 1-cm controlled cut in the lung surface. Animals served as control, or tetracycline was administered. The chest tube was removed after the air leak stopped. Animals were sacrificed after 20 days. Group A was control rabbits (6); group B had powdered tetracycline placed at thoracotomy; group C, tetracycline solution, 2 ml/kg, 25 mg/ml (4); and group D, tetracycline solution, 1 ml/kg, 50 mg/ml (10). Control rabbits showed few adhesions, and the pleura appeared to be normal by histologic examination. There was a spectrum of results with tetracycline, but with the concentrated tetracycline solution (group D) histologic examination showed uniform pleurodesis and thickening of the pleura. In group D it took no longer for the pulmonary air leak to seal than with the group A (control) rabbits. We conclude that concentrated tetracycline solution is effective in causing pleurodesis even when an active air leak is present. The lung, however, must be kept expanded so that symphysis can occur between the visceral and parietal pleura.
在一个制造了存在活动性漏气的气胸的动物模型中，尝试使用硬化剂进行胸膜固定术。成年兔子做了小的左胸切开术，然后在肺表面做一个1厘米的可控切口。动物被分为对照组，或给予四环素。漏气停止后拔除胸管。20天后处死动物。A组为对照兔子（6只）；B组在开胸手术时放置四环素粉末；C组，给予2毫升/千克、25毫克/毫升的四环素溶液（4只）；D组，给予1毫升/千克、50毫克/毫升的四环素溶液（10只）。对照兔子显示出很少的粘连，组织学检查显示胸膜外观正常。使用四环素出现了一系列结果，但使用浓缩四环素溶液（D组）时，组织学检查显示胸膜固定均匀且胸膜增厚。在D组中，肺漏气封闭所需时间并不比A组（对照）兔子长。我们得出结论，即使存在活动性漏气，浓缩四环素溶液在引起胸膜固定方面也是有效的。然而，肺必须保持膨胀，以便脏层和壁层胸膜之间能够发生粘连。
