通过气相色谱法、质谱法、等速电泳法和液相色谱法对尿毒症“中分子”组分进行表征。

Characterization of uremic "middle molecular" fractions by gas chromatography, mass spectrometry, isotachophoresis, and liquid chromatography.

作者信息

Schoots A C, Mikkers F E, Claessens H A, De Smet R, Van Landschoot N, Ringoir S M

出版信息

Clin Chem. 1982 Jan;28(1):45-9.

Abstract

Uremic ultrafiltrates (and normal serum, for comparison) were fractionated by means of gel filtration. The collected fractions were further investigated by combined analytical techniques: "high-performance" liquid chromatography, gas chromatography, mass spectrometry, and isotachophoresis. Ultrafiltrate fractions in the so-called middle molecular mass region (Mr 500-2000) contained a considerable amount of substances of low molecular mass, such as carbohydrates, organic acids, amino acids, and ultraviolet absorbing solutes. Ultraviolet absorbance in the "middle molecular mass region" of the gel chromatogram is mainly due to the presence of these rather low-molecular-mass solutes. Therefore this signal is not a quantitative measure of molecules with a "middle" molecular mass.

摘要

采用凝胶过滤法对尿毒症超滤物（以及作为对照的正常血清）进行分级分离。通过“高效”液相色谱、气相色谱、质谱和等速电泳等联合分析技术对收集的级分进行进一步研究。所谓的中等分子量区域（Mr 500 - 2000）的超滤物级分含有大量低分子量物质，如碳水化合物、有机酸、氨基酸和紫外线吸收溶质。凝胶色谱图“中等分子量区域”的紫外线吸光度主要归因于这些分子量相当低的溶质的存在。因此，该信号并非是对“中等”分子量分子的定量测量。