Althaus Z R, Rowland J M, Freeman J P, Slikker W
J Chromatogr. 1982 Jan 8;227(1):11-23. doi: 10.1016/s0378-4347(00)80351-5.
A high-performance liquid chromatography (HPLC) technique was developed for the determination of radiolabeled triamcinolone acetonide (TAC), cortisol and their metabolites in rhesus monkey plasma, urine and tissue samples. After protein precipitation, the parent compounds and metabolites were simultaneously resolved using a single-column reversed-phase HPLC system. TAC was subsequently verified by mass spectrometry and TAC glucuronide was tentatively identified by enzymatic hydrolysis and mass spectrometry of the hydrolysis product. The endogenous hormones, cortisol and cortisone were presumptively identified by cochromatography with authentic standards on two different HPLC systems and positively identified by reverse-isotope recrystallization. Other metabolites of both compounds were detected by selective enzymatic hydrolysis and HPLC. This method is rapid and reproducible with a total recovery greater than 80%.
开发了一种高效液相色谱（HPLC）技术，用于测定恒河猴血浆、尿液和组织样品中放射性标记的曲安奈德（TAC）、皮质醇及其代谢物。经过蛋白沉淀后，使用单柱反相HPLC系统同时分离母体化合物和代谢物。随后通过质谱法验证了TAC，并通过对水解产物进行酶水解和质谱法初步鉴定了TAC葡糖醛酸苷。通过在两种不同的HPLC系统上与标准品共色谱法推测鉴定内源性激素皮质醇和可的松，并通过反向同位素重结晶法进行确证。通过选择性酶水解和HPLC检测这两种化合物的其他代谢物。该方法快速且可重复，总回收率大于80%。
