Abidi S L
J Chromatogr. 1982 Jan 1;234(1):187-200. doi: 10.1016/s0021-9673(00)81792-6.
High-performance liquid chromatographic (HPLC) conditions are presented for the separation and quantitative determination of a homologous antibiotic complex (antimycin A). Combined HPLC and chemical ionization mass spectrometry proved to be exceptionally useful for the structural identification of chromatographic components. Using electrochemical, fluorescence, and ultraviolet detectors, the minimum detectable amounts of the antibiotics were found to be in the ranges 0.10-1.12, 0.31-1.69, and 4.10-28.2 ng, respectively. Advantages of the preparation of Dns derivatives for use in fluorescence detection are discussed. Application of the HPLC technique to the analysis of the antibiotic mixture in organic tissues is demonstrated.
本文介绍了用于分离和定量测定同源抗生素复合物（抗霉素A）的高效液相色谱（HPLC）条件。HPLC与化学电离质谱联用被证明对色谱组分的结构鉴定极为有用。使用电化学、荧光和紫外检测器时，发现抗生素的最低检测量分别在0.10 - 1.12、0.31 - 1.69和4.10 - 28.2 ng范围内。讨论了制备用于荧光检测的丹磺酰氯（Dns）衍生物的优点。展示了HPLC技术在有机组织中抗生素混合物分析中的应用。
