Vukovich T, Koller E, Doleschel W
Thromb Haemost. 1978 Jun 30;39(3):600-6.
An investigation of the influence of thrombin on the clotting activity of factor VIII was made. Purified factor VIII and different amounts of thrombin complexed to Sepharose 4 B were mixed and incubated for various periods of time. The factor VIII activities of these incubation mixtures were determined by the one- and two-stage analytical procedures in the presence of the thrombin-sepharose and in its absence following the latter removal from the test sample by filtration. The results so obtained confirm the view that thrombin inactivates factor VIII. Evidences for a thrombin-induced potentiation of the factor VIII activity, seen only in the thrombin-sepharose containing test samples analyzed by the one-stage method, are here interpreted as thrombin-effects peculiar to this factor VIII test system and not as potentiation by thrombin of the factor itself.
开展了一项关于凝血酶对凝血因子 VIII 凝血活性影响的研究。将纯化的凝血因子 VIII 与不同量的与琼脂糖 4B 复合的凝血酶混合，并在不同时间段进行孵育。通过单阶段和双阶段分析程序，在存在凝血酶 - 琼脂糖的情况下以及在通过过滤从测试样品中去除凝血酶 - 琼脂糖后不存在的情况下，测定这些孵育混合物中凝血因子 VIII 的活性。所获得的结果证实了凝血酶使凝血因子 VIII 失活的观点。仅在通过单阶段方法分析的含凝血酶 - 琼脂糖的测试样品中观察到的凝血酶诱导的凝血因子 VIII 活性增强的证据，在此被解释为该凝血因子 VIII 测试系统特有的凝血酶效应，而不是凝血酶对该因子本身的增强作用。
