Zeindl E, Sperling K, Klose J
Mutat Res. 1982 Feb;97(1):67-78. doi: 10.1016/0165-1161(82)90020-6.
Chemical mutagenesis was studied on Chinese hamster ovary cells by protein mapping. Cell cultures were treated with methylnitrosourea and the cells were cloned in non-selective media. The proteins of single clones were separated by 2-dimensional electrophoresis and analysed for qualitative (electrophoretic mobility) and quantitative (staining intensity; presence/absence) changes in the protein patterns. The investigation included 26 clones derived from treated cells and 26 control clones. The total number of gene loci tested was calculated from the number of protein spots analysed: it amounted to about 33 800. The protein patterns revealed 2 alterations defined as qualitative variant proteins. No alteration of this type was found in the control group. The frequency of quantitative variant proteins was increased by more than 100% compared with the control group. Our results and theoretical considerations suggest that the cellular concentration of single proteins offers a sensitive parameter for mutagenicity testing.
通过蛋白质图谱研究了化学诱变对中国仓鼠卵巢细胞的影响。细胞培养物用甲基亚硝基脲处理，然后将细胞接种于非选择性培养基中进行克隆。通过二维电泳分离单个克隆的蛋白质，并分析蛋白质图谱中的定性（电泳迁移率）和定量（染色强度；存在与否）变化。该研究包括26个来自处理细胞的克隆和26个对照克隆。根据分析的蛋白质斑点数量计算所测试基因座的总数：总计约33800个。蛋白质图谱显示有2种改变被定义为定性变异蛋白。对照组未发现此类改变。与对照组相比，定量变异蛋白的频率增加了100%以上。我们的结果和理论思考表明，单一蛋白质的细胞浓度为致突变性测试提供了一个敏感参数。
