Harano T, Harano K, Yamasaki K
Steroids. 1978 Jul-Aug;32(1):73-83. doi: 10.1016/0039-128x(78)90101-0.
The conventional methods of gas liquid chromatography or mass spectrometry failed to be useful for the identification of the biliary 3beta,7alpha-dihydroxychol-5-en-24-oic acid, a key intermediate of chenodeoxycholic acid biogenesis. It has been preliminarily reported that this acid in human bile was successfully identified by gas chromatography-mass spectrometry, after the methoxylation of its allyl alcohol group. Physical as well as spectral properties of the methoxylation products derived from the acid were reported, compared with those from its 7beta-epimer.
传统的气相液相色谱法或质谱法无法用于鉴定鹅去氧胆酸生物合成的关键中间体——胆汁中的3β,7α-二羟基胆-5-烯-24-酸。初步报道称，在其烯丙醇基团进行甲氧基化后，通过气相色谱-质谱法成功鉴定了人胆汁中的这种酸。报告了该酸衍生的甲氧基化产物的物理和光谱性质，并与来自其7β-差向异构体的产物进行了比较。
