Golovatskiĭ I D, Grivnak L M, Gashpar E D
Ukr Biokhim Zh (1978). 1982;54(1):21-5.
The activity of lactate dehydrogenase from the cattle myocardium extract as well as of this enzyme crystalline form was studied in the presence of structural analogues of the nicotinamide fragment NAD-amide, diethyl amide and N-oxymethyl amide of nicotinic acid. It is shown that the rate of the reaction catalyzed with lactate dehydrogenase under the effect of amide and N-oxymethyl amide of nicotinic acid decreases to a different extent depending on their concentration. Nicotinic diethyl amide practically has no effect on the activity of lactate dehydrogenase. Ki for crystalline lactate dehydrogenase and myocardium lactate dehydrogenase in the presence of nicotinic amide is 0.23.10-3 and 1.13.10-3 M. respectively, in the presence of nicotinic N-oxymethyl amide-0.30.10-3 and 0.10.10-3 M.
在烟酰胺片段NAD - 酰胺、烟酸二乙酰胺和N - 氧甲基酰胺的结构类似物存在的情况下，研究了牛心肌提取物中乳酸脱氢酶的活性以及该酶结晶形式的活性。结果表明，在烟酸酰胺和N - 氧甲基酰胺的作用下，乳酸脱氢酶催化的反应速率根据其浓度不同程度地降低。烟酸二乙酰胺实际上对乳酸脱氢酶的活性没有影响。在烟酰胺存在下，结晶乳酸脱氢酶和心肌乳酸脱氢酶的Ki分别为0.23×10⁻³和1.13×10⁻³ M，在烟酸N - 氧甲基酰胺存在下分别为0.30×10⁻³和0.10×10⁻³ M。
