Roy S K, Sengupta J, Paria B C, Manchanda S K
Acta Endocrinol (Copenh). 1982 Jan;99(1):129-35. doi: 10.1530/acta.0.0990129.
Rat blastocyst development and expansion were studied in an in vitro culture system. Early blastocysts collected from rats on the morning of day 5 were shown to develop normally and underwent expansion when placed in bovine serum albumin supplemented culture medium for 12 h at 37 degrees C. Nafoxidine, an oestrogen antagonist, prevented embryo expansion when added to the culture medium, and it was found that if oestradiol-17 beta was added to this medium this inhibitory effect was removed and the embryos again expanded. Ethanol, used as a solvent for steroids, and cholesterol were added to the medium containing nafoxidine but the blastocysts remained unexpanded after 12 h in culture. These results suggest a need for oestrogen action during blastocyst expansion in vitro. Cultured rat blastocysts were subjected to histochemical study for acid phosphatase (ACPase) and leucylnaphthylamidase (LNAse). In unexpanded blastocysts ACPase was found to be low while LNAse activity was higher when compared with enzyme distribution patterns in expanded blastocysts.
在体外培养系统中研究了大鼠囊胚的发育和扩张。从第5天上午的大鼠中收集的早期囊胚在置于补充有牛血清白蛋白的培养基中于37℃培养12小时后，显示出正常发育并发生了扩张。雌激素拮抗剂萘福昔定添加到培养基中时可阻止胚胎扩张，并且发现如果将17β-雌二醇添加到该培养基中，这种抑制作用就会消除，胚胎会再次扩张。用作类固醇溶剂的乙醇和胆固醇添加到含有萘福昔定的培养基中，但培养12小时后囊胚仍未扩张。这些结果表明在体外囊胚扩张过程中需要雌激素发挥作用。对培养的大鼠囊胚进行了酸性磷酸酶（ACPase）和亮氨酰萘基酰胺酶（LNAse）的组织化学研究。与扩张囊胚中的酶分布模式相比，未扩张囊胚中的ACPase含量较低，而LNAse活性较高。
