Meyer R A, Kuchmerick M J, Brown T R
Am J Physiol. 1982 Jan;242(1):C1-11. doi: 10.1152/ajpcell.1982.242.1.C1.
This review presents the principles and limitations of phosphorus nuclear magnetic resonance (31P-NMR) spectroscopy as applied to the study of striated muscle metabolism. Application of the techniques discussed include noninvasive measurement of high-energy phosphate, intracellular pH, intracellular free Mg2+, and metabolite compartmentation. In perfused cat biceps (fast-twitch) muscle, but not in soleus (slow-twitch), NMR spectra indicate a substantially lower (1 mM) free inorganic phosphate level than when measured chemically (6 mM). In addition, saturation and inversion spin-transfer methods that enable direct measurement of the unidirectional fluxes through creatine kinase are described. In perfused cat biceps muscle, results suggest that this enzyme and its substrates are in simple chemical equilibrium.
本综述介绍了磷核磁共振（31P-NMR）光谱技术用于研究横纹肌代谢的原理和局限性。所讨论技术的应用包括对高能磷酸、细胞内pH值、细胞内游离Mg2+和代谢物分隔的无创测量。在灌注的猫肱二头肌（快肌）中，而非比目鱼肌（慢肌）中，NMR光谱显示游离无机磷酸盐水平（1 mM）显著低于化学测量值（6 mM）。此外，还描述了能够直接测量通过肌酸激酶的单向通量的饱和和反转自旋转移方法。在灌注的猫肱二头肌中，结果表明该酶及其底物处于简单的化学平衡状态。
