Javitt N B, Kok E, Lloyd J, Benscath A, Field F H
Biomed Mass Spectrom. 1982 Feb;9(2):61-3. doi: 10.1002/bms.1200090203.
To further studies of the metabolism of 26-hydroxycholesterol in fetal and neonatal life, a deuterated compound was prepared from kryptogenin by Clemmensen reduction. The spectra of the deuterated 26-hydroxycholesterol showed that five to nine deuterium atoms were incorporated per 26-hydroxycholesterol molecule, with the maximum incorporation of eight deuterium atoms. The deuterated compound was recovered unchanged from the feces of a child following oral administration. Comparison of the ratio of deuterated to protium compound indicated the presence of an endogenous pool of 26-hydroxycholesterol. Parenteral administration of the compound to a hamster indicated metabolism to deuterated chenodeoxycholic acid. The compound is useful as an isotope tracer for studies of the endogenous metabolism of 26-hydroxycholesterol.
为了进一步研究26-羟基胆固醇在胎儿和新生儿期的代谢情况，通过克莱门森还原法从隐甾醇制备了一种氘代化合物。氘代26-羟基胆固醇的光谱显示，每个26-羟基胆固醇分子中掺入了5至9个氘原子，最大掺入量为8个氘原子。口服给药后，该氘代化合物在一名儿童的粪便中未发生变化地被回收。氘代与普通氢化合物的比例比较表明存在内源性26-羟基胆固醇池。对仓鼠进行该化合物的肠胃外给药表明其代谢为氘代鹅去氧胆酸。该化合物可用作研究26-羟基胆固醇内源性代谢的同位素示踪剂。
