Kuttab S H, Tanglertpaibul S, Vouros P
Biomed Mass Spectrom. 1982 Feb;9(2):78-84. doi: 10.1002/bms.1200090208.
The mass spectrometric properties of procarbazine and some of its breakdown products were examined using isotopic labeling and high resolution mass spectrometry. A specific intramolecular methyl group transfer, induced by electron impact, was used diagnostically to distinguish between two isomeric derivatives. The mass spectrometric information was used for identification of the in vivo metabolites of procarbazine in rat. Minimum sample manipulation was used due to the inherent instability of the suspected metabolites. The azo compound N-isopropyl- alpha -(2-methylazo)-p-toluamide was identified as the principal circulating metabolite of procarbazine.
使用同位素标记和高分辨率质谱法研究了丙卡巴肼及其一些分解产物的质谱特性。利用电子轰击诱导的特定分子内甲基转移进行诊断，以区分两种同分异构衍生物。质谱信息用于鉴定大鼠体内丙卡巴肼的代谢产物。由于可疑代谢产物固有的不稳定性，尽量减少了样品处理。偶氮化合物N-异丙基-α-(2-甲基偶氮)-对甲苯酰胺被鉴定为丙卡巴肼的主要循环代谢产物。
